High-quality stem cell culture supernatant
Stem cell culture supernatant treatment is expected to be a next-generation regenerative medicine technology as a cell-free therapy. However, although it is much cheaper than cell therapy, the current situation is that the price is not so low that anyone can receive it. The reason for this is that the medium for cell culture and the necessary reagents are expensive.
Therefore, by developing a stem cell supernatant containing growth factors, cytokines, and exosomes at a higher concentration than the current level, we are conducting research and development with the aim of disseminating treatments that can achieve the same effect at a lower price. I’m here.
In general, cell cultures have been two-dimensionally (2D, flat) cultured in a plastic container for tissue culture or adhered to an extracellular matrix (hereafter referred to as ECM). Under physiological conditions, cells constantly interact with the ECM, which controls complex biological functions such as cell migration, apoptosis, transcriptional regulation, and receptor expression. In cells cultured in a two-dimensional environment, complex signal exchanges between cells and ECM cannot be reproduced, so it is considered impossible to directly reflect experimental results of 2D culture in clinical trials.
Three-dimensional (3D) cell culture overcomes these problems and more closely resembles the in vivo physiological environment, and several studies have demonstrated that gene and protein expression in cells cultured in a 3D environment It has been reported that the profile is different from that of 2D culture, and the expression profile under 3D culture environment is of much higher physiological importance compared to 2D culture environment.
Since stem cells are also a major factor that affects exosomes, which are physiologically active substances produced by stem cells, we developed a method of culturing stem cells in 3D instead of 2D when producing supernatant fluid and exosomes. We are conducting research to produce higher quality supernatants with the aim of producing bioactive substances and exosomes in a state closer to the in vivo state.